Release of CGRP in vivo from rat dura mater: Influence of capsaicin and topiramate

Introduction Migraine is a disease that stands out for its high prevalence with changes in the nervous system, abnormal levels of neurotransmitters, neuromodulators, and neuropeptides. The main mechanisms action capsaicin are chemical induction through activation TRPV1 channels, allowing calcium influx into neurons trigeminal ganglion dura mater, activating mast cell degranulation, releasing pro-inflammatory (e.g., histamine, oxide nitric) vasoactive CGRP substance P) substances. For treatment, classes drugs used to act on blood vessels prevent vasodilation, as well depolarization sensory fibers mater. Objectives To better understand sterile inflammation after exposing mater bilaterally, we created an experimental model study topiramate degranulation release rat skull preparation vivo using anesthetized animals. Methods Thirty-five Wistar rats were used, divided two groups chronic (GTC) treated 20mg/kg/day, gavage/10 days, acute (GTA) situ (10-3M). animals cranial windows between coronal lambda sutures hemicraniums performed drill expose bilaterally. 10-3M was placed right side synthetic interstitial fluid left exposed contact 10 minutes small cotton ball soaked respective solutions so there no leakage posteriorly kept freezer (-20°C) later quantification CGRP. percentage degranulated cells quantified removal by staining it toluidine blue. A commercial enzyme immunoassay from mater... (Too see complet abstract, please, check PDF.)